Chitosan/Carboxymethyl Cellulose Polyelectrolyte Complex Scaffolds for Pulp Cells Regeneration

Author:

Chen Huangqin1,Fan Mingwen2

Affiliation:

1. Key Laboratory for Oral Biomedical Engineering, Ministry of Education School of Stomatology, Wuhan University, Wuhan 430079, PR China

2. Key Laboratory for Oral Biomedical Engineering, Ministry of Education School of Stomatology, Wuhan University, Wuhan 430079, PR China,

Abstract

Novel polyelectrolyte complex (PEC) chitosan/carboxymethyl cellulose (CMC) scaffolds are prepared using a freeze-dry process. The microstructure is characterized by SEM and the average pore diameters and internal porosities are calculated. The morphology and distribution of pulp cells on these three-dimensional scaffolds are investigated by SEM and confocal laser scanning microscopy (CLSM). The expression of osteonectin (ON) and dentin sialophosphoprotein (DSPP) is detected by reverse transcription polymerase chain reaction (RT-PCR). The scaffolds are then implanted subcutaneously into BALB/c mice. The addition of CMC to chitosan decreases the pore diameter and increases internal porosity. The pulp cells co-cultured in these PEC scaffolds show improved adhesion, spreading, cell capacity, and three-dimensional configurations compared to pure chitosan scaffolds. Based on the data obtained, the chitosan/CMC complex provides an improved scaffold material for pulp tissue engineering.

Publisher

SAGE Publications

Subject

Materials Chemistry,Polymers and Plastics,Biomaterials,Bioengineering

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