Polyionic Hydrogels Obtained by Complexation between Xanthan and Chitosan: Their Properties as Supports for Enzyme Immobilization

Author:

Dumitriu S.1,Magny P.1,Montané D.1,Vidal P.F.1,Chornet E.1

Affiliation:

1. Department of Chemical Engineering University of Sherbrooke Sherbrooke, Quebec, Canada J1K 2R1

Abstract

A method for the preparation of hydrogels from the complexation of chitosan and xanthan is reported. Stable hydrogels capable of retaining be tween 65 and 95% weight water were prepared. The water retention and prop erties of the hydrogels were studied as a function of the degree of acetylation of chitosan and the ratio chitosan/xanthan used in the preparation of the gel. Spectroscopic FTIR was used to confirm complexation between the amine (chi tosan) and carboxylic (xanthan) groups. Electron micrographs (SEM and TEM) show the formation of a fibrillar structure with characteristic pore sizes be tween 100 and 1000 nm and fibril diameters between 50 and 100 nm. The diffu sion coefficient of 4- O-methyl- d-glucurono-D-xylan Remazol Brilliant Blue R (RBB-xylan) in the complex chitosan-xanthan was 2.02 × 10-12 m 2s-1 at 30°C. The chitosan-xanthan complex was used to immobilize two enzymes (endo-1,4- β-xylanase and protease) either as single enzymes or as a binary system. Immo bilization varied between 85 and 98%. The immobilized xylanase activity was significantly greater with respect to the free enzyme while the binary enzyme system promoted protease activity.

Publisher

SAGE Publications

Subject

Materials Chemistry,Polymers and Plastics,Biomaterials,Bioengineering

Reference51 articles.

1. Burgos, H. 1989. Chitin and Chitosan, S. B. Gudmund, T. Anthonsen and P. Sandford, eds. London and New York: Elsevier Appl. Sci., p. 573.

2. Dumitriu, S. 1992. High Performance Biomaterials, M. Szycher, ed. Lancaster, PA: Technomic Publishing Co., Inc., p. 669.

3. Bioactive polymers 65—studies of cross-linked xanthan hydrogels as supports in drug retardation

4. Peppas, N.A. and A.G. Mikos. 1986. Hydrogels in Medicine and Pharmacy, Vol. I, Fundamentals, N. A. Peppas, ed. Boca Raton, FL: CRC Press, Inc., pp. 2-24.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3