An efficient functionalization of dexamethasone-loaded polymeric scaffold with [3-(2,3-epoxypropoxy)-propyl]-trimethoxysilane coupling agent for bone regeneration: Synthesis, characterization, and in vitro evaluation

Abstract

In this study, dexamethasone-loaded gelatin–starch scaffolds were fabricated by the freeze-drying technique under different cooling temperatures and polymeric compositions. The constructs were modified via [3-(2,3-epoxypropoxy)-propyl]-trimethoxysilane coupling agent in order to produce a bioactive network structure for bone tissue engineering applications. Herein, the synergistic effect of [3-(2,3-epoxypropoxy)-propyl]-trimethoxysilane and dexamethasone was examined on the bioactivity and osteogenic behavior of scaffolds. Based on scanning electron microscopy micrographs, more fine pores were formed at higher freezing temperatures. The prepared microstructure at a rapid freezing rate resulted in diminished mechanical properties and a greater level of swelling and durability compared with a slow freezing rate. According to the acquired results, the mechanical strength decreased, while both absorption capacity and mass loss rate increased as a function of starch addition. Furthermore, the enhancement of hydrophilicity and reduction of mechanical stability enhanced the dexamethasone release levels. In addition, the synthesized constructs confirmed the positive effect of [3-(2,3-epoxypropoxy)-propyl]-trimethoxysilane and dexamethasone on biomimetic mineralization of the scaffolds. Supporting the cellular adhesion and proliferation alongside the expression of alkaline phosphatase, especially in the presence of dexamethasone, was the other advantage of synthetic scaffolds as a bone reconstructive substitute. Accordingly, drug-loaded hybrid constructs seem to be promising for further preclinical and clinical investigations in bone tissue engineering.