Impact of IL-33 on PGD2 Generation by Activated Human Cord Blood-Derived Mast Cell: Lack of Effect on Tryptase Release

Author:

Nicoletti M.1,Maccauro G.2,Tripodi D.3,Saggini A.4,Potalivo G.5,Castellani M.L.6,Conti F.7,Rosati M.7,Tomato E.6,Caraffa A.5,Antinolfi P.5,Conti P.6,Theoharides T.C.8

Affiliation:

1. Department of Neurosciences and Imaging, University of Chieti, Italy

2. Orthopedics Division, Catholic University of Rome, Rome, Italy

3. Dental School, University of Chieti-Pescara, Chieti, Italy

4. Dermatology Department, University Tor Vergata, Rome, Italy

5. Orthopedics Division, University of Perugia, Perugia, Italy

6. Immunology Division, University of Chieti-Pescara, Chieti, Italy

7. Gynecology Division, “Santo Spirito” Hospital, Pescara, Italy

8. Department of Physiology and Pharmacology, Tufts University School of Medicine, New England Medical Center, Boston, MA, USA

Abstract

Mast cells are important not only in allergic reactions, but also in inflammation and are involved in a variety of responses including the immediate release of potent inflammatory mediators after activation by cross-linking of FcεRI molecules. Prostaglandin D2 (PGD2) is a major cyclooxygenase metabolite of arachidonic acid produced by mast cells and it is released following allergen challenge in allergic diseases. IL-33 is an iflammatory cytokine which is critically involved in the regulation of in vitro and in vivo cyclooxygenase production, providing a potential therapeutic target for inflammatory disorders. In this study, using human derived umbelical cord blood mast cells, we show that IL-33 (50 ng/ml), and calcium ionophore A 23187 (0.5 μg/ml), compound 48/80 (10−5 M) or anti-IgE (10 μg/ml), enhaced the production of PGD2 and this effect was inhibited by indomethacin. However, IL-33 was unable to induce tryptase release in these cells. These effects confirm the inflammatory property of IL-33 by stimulating PGD2 but not tryptase in human mast cells. The inhibitory effect of this new cytokine may have a potential therapeutic response in allergic and inflammatory diseases.

Publisher

SAGE Publications

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Mast Cell Cytokines in Acute and Chronic Gingival Tissue Inflammation: Role of IL-33 and IL-37;International Journal of Molecular Sciences;2022-10-31

2. Targeting IL-33 in Autoimmunity and Inflammation;Journal of Pharmacology and Experimental Therapeutics;2015-04-23

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