Homogenous and Microbeam X-Ray Radiation Induces Proteomic Changes in the Brains of Irradiated Rats and in the Brains of Nonirradiated Cage Mate Rats-Reference-Cited by-同舟云学术

Homogenous and Microbeam X-Ray Radiation Induces Proteomic Changes in the Brains of Irradiated Rats and in the Brains of Nonirradiated Cage Mate Rats

Published:2018-01-01 Issue:1 Volume:16 Page:155932581775006
ISSN:1559-3258
Container-title:Dose-Response
language:en
Short-container-title:Dose-Response

Author:

Smith Richard¹^ORCID,Wang Jiaxi²,Seymour Colin¹,Fernandez-Palomo Cristian¹^ORCID,Fazzari Jennifer¹,Schültke Elisabeth³,Bräuer-Krisch Elke⁴,Laissue Jean⁵,Schroll Christian⁶,Mothersill Carmel¹

Affiliation:

1. Department of Medical Physics and Applied Radiation Sciences, McMaster University, Hamilton, Ontario, Canada

2. Mass Spectrometry Facility, Department of Chemistry, Queen’s University, Kingston, Ontario, Canada

3. Department of Radio-oncology, Rostock University Medical Centre, Rostock, Germany

4. European Synchrotron Radiation Facility, Grenoble, France

5. Institute of Pathology, University of Bern, Bern, Switzerland

6. Stereotactic Neurosurgery and Laboratory for Molecular Neurosurgery, Freiburg University Medical Centre, Freiburg, Germany

Abstract

To evaluate microbeam radiation therapy (MRT), for brain tumor treatment, the bystander effect in nonirradiated companion animals was investigated. Adult rats were irradiated with 35 or 350 Gy at the European Synchrotron Research Facility using homogenous irradiation (HR) or MRT to the right brain hemisphere. The irradiated rats were housed with nonirradiated rats. After 48 hours, all rats were euthanized and the frontal lobe proteome was analyzed using 2-dimensional electrophoresis and mass spectrometry. Proteome changes were determined by analysis of variance ( P < .05). Homogenous irradiation increased serum albumin, heat shock protein 71 (HSP-71), triosephosphate isomerase (TPI), fructose bisphosphate aldolase (FBA), and prohibitin and decreased dihydrolipoyl dehydrogenase (DLD) and pyruvate kinase. Microbeam radiation therapy increased HSP-71, FBA, and prohibitin, and decreased aconitase, dihydropyrimidinase, TPI, tubulin DLD, and pyruvate kinase. Cage mates with HR irradiated rats showed increased HSP-71 and FBA and decreased pyruvate kinase, DLD, and aconitase. Cage mates with MRT irradiated rats showed increased HSP-71, prohibitin, and FBA and decreased aconitase and DLD. Homogenous irradiation proteome changes indicated tumorigenesis, while MRT proteome changes indicated an oxidative stress response. The bystander effect of proteome changes appeared antitumorigenic and inducing radioresistance. This investigation also supports the need for research into prohibitin interaction with HSP-70/71 chaperones and cancer therapy.

Funder

Canada Research Chairs

Publisher

SAGE Publications

Subject

Chemical Health and Safety,Health, Toxicology and Mutagenesis,Public Health, Environmental and Occupational Health,Toxicology

Link

http://journals.sagepub.com/doi/pdf/10.1177/1559325817750068

Reference70 articles.

1. Effects of pulsed, spatially fractionated, microscopic synchrotron X-ray beams on normal and tumoral brain tissue

2. SYRA3 COST Action – Microbeam radiation therapy: Roots and prospects

3. Applications of synchrotron X-rays to radiotherapy