Role of M Cells and Macrophages in the Entrance ofMycobacterium paratuberculosisinto Domes of Ileal Peyer's Patches in Calves

Author:

Momotani E.1,Whipple D. L.1,Thiermann A. B.1,Cheville N. F.1

Affiliation:

1. US Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Ames, IA

Abstract

Ligated ileal loops of calves were inoculated with live and heat-killed Mycobacterium paratuberculosis and were examined by light and electron microscopy. At 5 hours after inoculation, acid-fast bacilli were in subepithelial macrophages, but not in M cells covering domes. At 20 hours, more than 50 acid-fast bacilli per cross section were in subepithelial macrophages in domes. Both living and heat-killed bacilli passed into domes. Addition of anti- M. paratuberculosis bovine scrum to the inoculum enhanced entry of bacteria into domes. By electron microscopy, intact bacilli with electron-transparent zones (peribacillary spaces) were in the supranuclear cytoplasm of M cells at 20 hours. M cells also contained vacuoles, including electron-dense material interpreted as degraded bacilli. Subepithelial and intraepithelial macrophages contained bacilli and degraded bacterial material in phagosomes. These results suggest that calf ileal M cells take up bacilli, and that subepithelial and intraepithelial macrophages secondarily accept bacilli or bacterial debris which are expelled from M cells.

Publisher

SAGE Publications

Subject

General Veterinary

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