Morphometry of Equine Neutrophils Isolated at Different Temperatures

Abstract

Equine neutrophils were evaluated ultrastructurally and by morphometric analysis. Homogeneous populations of neutrophils were isolated from peripheral blood at 4° and 22°C by centrifugation on two sequential Ficoll-Hypaque density gradients. Isolation procedures at both temperatures resulted in neutrophil degranulation but not cell swelling. Degranulation was more extensive in cells isolated at 22°C. Isolation temperature affected the neutrophil content of secondary granules more than primary granules. A granule similar to immature specific granules of human neutrophils was observed. Granules with a flocculent matrix were more frequent in cells processed at 22°C. These granules were considered to be involved in the degranulation process.