A Histochemical Study of Changes Observed in the Mouse Diaphragm after Organophosphate Poisoning-Reference-Cited by-同舟云学术

A Histochemical Study of Changes Observed in the Mouse Diaphragm after Organophosphate Poisoning

Published:1991-01 Issue:1 Volume:10 Page:9-14
ISSN:0960-3271
Container-title:Human & Experimental Toxicology
language:en
Short-container-title:Hum Exp Toxicol

Author:

Bright J.E.¹,Inns R.H.¹,Tuckwell N.J.¹,Griffiths G.D.¹,Marrs T.C.²

Affiliation:

1. CDE Porton Down, Salisbury, Wilts SP4 0JQ, UK

2. Present address: Department of Health, Hannibal House, Elephant and Castle, London SE1 6TE, UK

Abstract

A sublethal dose of sarin (GB, isopropyl methylphosphonofluoridate) was administered to mice. The animals were killed up to 28 d after dosing and frozen sections were made of the excised diaghragms which were stained using haematoxylin and eosin and a modified Gomori trichrome method. Muscle fibre degeneration and mononuclear infiltration were seen, notably at 24 h and 3 d. A number of histochemical procedures were carried out, including the GBHA procedure for ionized calcium. Calcium accumulation, seen at 4 h, was the earliest abnormality observed. All changes were rapidly regressing by 5 d and histological appearances were normal by 14 d. It was concluded that sarin produced myopathic changes preceded by calcium accumulation.

Publisher

SAGE Publications

Subject

Health, Toxicology and Mutagenesis,Toxicology,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/096032719101000102

Reference27 articles.

1. Reversible necrosis at the end-plate region in striated muscles of the rat poisoned with cholinesterase inhibitors

2. Agonist-induced myopathy at the neuromuscular junction is mediated by calcium.

3. Pesticide induced muscle necrosis: Mechanisms and prevention*1

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