Circle RNA circCSPP1 promotes human osteosarcoma cell proliferation and increases glucose metabolism by suppressing miR-200c maturation

Author:

Zhang L1,Yang ST2,Wang C2,Zhang LC2,Zhang X1,Li FC2,Wang SY2ORCID,Ma K2ORCID

Affiliation:

1. Experiment Center of Basic Medical Sciences of Kunming Medical University, Kunming City, P. R. China

2. Department of Orthopedics, The First Affiliated Hospital of Kunming Medical University, Kunming City, P. R. China

Abstract

Introduction MiR-200c plays a central role in glucose metabolism in cancer cells. However, its upstream regulators in this process are unknown. CircRNA CSPP1 (circCSPP1) was predicted to bind to premature miR-200c, an oncogenic miRNA. Therefore, we explored their interaction in osteosarcoma (OS). Methods Differential circCSPP1 and miR-200c expression in OS was analyzed using RT-qPCR. Glucose metabolism was analyzed by glucose uptake assay. Subcellular circCSPP1 location in OS cells was detected using cellular fractionation assay. The direct interaction between circCSPP1 and miR-200c was explored using RNA-RNA pull-down assay. The role of circCSPP1 in miR-200c maturation was investigated by analyzing both mature and premature miR-200c levels in OS cells with circCSPP1 overexpression. Results CircCSPP1 and premature miR-200c levels were increased while mature miR-200c level was decreased in OS. CircCSPP1 was detected in both the nuclear and cytoplasm fractions of OS cells. CircCSPP1 directly interacted with premature miR-200c. CircCSPP1 overexpression increased premature miR-200c level, glucose uptake, and cell proliferation, but decreased mature miR-200c level. MiR-200c overexpression suppressed the role of circCSPP1 in OS cells. Conclusions CircCSPP1 promotes OS cell proliferation and increases glucose metabolism by suppressing miR-200c maturation.

Funder

Yunnan Provincial Bone and Joint Disease Clinical Medicine Center Project

Publisher

SAGE Publications

Subject

Health, Toxicology and Mutagenesis,Toxicology,General Medicine

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