Myocardial protective effect of sacubitril-valsartan on rats with acute myocardial infarction

Abstract

Objective: The purpose of this study was to explore the effect of sacubitril-valsartan on rats with acute myocardial infarction. Methods: Sprague-dawley rats were randomly divided into six groups. Rats in Group A and B were threaded without deligation and treated with valsartan (34 mg/kg) or sacubitril-valsartan (68 mg/kg) after operation. Rats in Group C and D were given the two drugs (34 mg/kg, 68 mg/kg) after ligation of the left anterior descending branch for 40 minutes. Rats in Group E and F were restored the blood of the coronary artery after ligation, and given the two drugs (34 mg/kg, 68 mg/kg) at the same time. N-terminal pro-brain natriuretic peptide, high sensitivity troponin T, aldosterone and Cyclic guanosine monophosphate were measured and Color Doppler echocardiography was performed. Six weeks later, the rats were killed, the hearts were weighed and stained with Masson staining. Results: Compared with Group A and B, the levels of N-terminal pro-brain natriuretic peptide, high sensitivity troponin T, aldosterone and Cyclic guanosine monophosphate in other groups were significantly increased ( p < 0.05). Before treatment, the left ventricular end diastolic diameter and left ventricular end systolic diameter were similar in each group. After treatment, the levels of left ventricular end diastolic diameter and left ventricular end systolic diameter, and collagen fiber range stained with blue in other groups were significantly increased in comparison with Group A and B ( p < 0.05). In addition, the left ventricular volume and collagen fiber range stained with blue were notably decreased, the levels of ejection fraction (EF) were increased in sacubitril-valsartan groups in comparison with valsartan groups ( p < 0.05). Conclusion: Early application of sacubitril-valsartan has a protective effect on rats with acute myocardial infarction.