Bio-degumming technology of Apocynum venetum bast by Pectobacterium sp. DCE-01

Abstract

Apocynum venetum fiber is known as the “king of wild fibers”. The traditional chemical degumming method for A. venetum limits the development of the industry due to serious pollution and fiber damage. By machine rolling preprocessing, bacteria culture, soaking fermentation, inactivation, water scrubbing, and drying under fermentation conditions of bath ratio 1:15, inoculum size 2%, temperature 33℃, and fermentation time 16 h, A. venetum fiber could be extracted from A. venetum bast. Strain Pectobacterium sp. DCE-01 simultaneously secreted pectinase, mannanase, and xylanase, which can decompose the main components of A. venetum non-cellulose. The catalytic activities of pectase, mannanase, xylanase, and cellulase at 16 h were 12.47, 7.56, 6.02, and 2.82 U/ml respectively. Compared with chemical degumming, bio-degumming exhibited 22.56% higher residual gum content, 18.14% higher breaking strength, and 44.33% lower chemical oxygen demand.