Indigo Backstaining During Cellulase Washing

Author:

Cavaco-Paulo Artur1,Morgado Jose2,Almeida Luis2,Kilburn Douglas3

Affiliation:

1. Departamento de Engenharia Têxtil. Universidade do Minho, Guimãres, Portugal, Biotechnology Laboratory, Department of Microbiology and Immunology, UBC, Vancouver, V6T 1Z3, Canada

2. CITEVE, V. N. Famalicão, Portugal

3. Biotechnology Laboratory, Department of Microbiology and Immunology, UBC, Vancouver, V6T 1Z3, Canada

Abstract

We have attempted to understand the mechanisms of indigo backstaining during enzyme stone washing by separately analyzing dye staining levels on different cotton surfaces during the process. The high ability of the cellulase enzyme protein to bind to cotton cellulose is the major cause of backstaining. Further studies of commercial cellulases show that the pH of maximum cellulase activity may not be the same pH of maximum of cellulase binding capacity to the substrate, and also that indigo dyes have completely different affinities for cellulase proteins from different fungal origins.

Publisher

SAGE Publications

Subject

Polymers and Plastics,Chemical Engineering (miscellaneous)

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