A Dehydration Method for Immature or Wet Cotton Fibers for Light and Electron Microscopy

Author:

Rajasekaran Kanniah1,Muir Alan J.2,Ingber Bruce F.2,French Alfred D.2

Affiliation:

1. USDA, ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, U.S.A.,

2. USDA, ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, U.S.A.

Abstract

A rapid dehydration procedure with acidified 2,2-dimethoxypropane (DMP) has been successfully applied to immature or wet cotton fibers. The ultrastructural integrity and fiber quality parameters such as cell wall area and perimeter of developing and mature cotton fibers were well-pre-served using this procedure as demonstrated by light, scanning and electron microscopy of whole fibers and cross-sections. Embedding in resin following DMP dehydration also prevented the collapse of the immature fibers due to drying of lumen.

Publisher

SAGE Publications

Subject

Polymers and Plastics,Chemical Engineering (miscellaneous)

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