LOCALIZATION OF A PRIMATE-SPECIFIC ESTERASE USING IMMUNOFLUORESCENCE AND IMMUNOPEROXIDASE TECHNIQUES

Abstract

The techniques of mixed antibody immunoperoxidase and indirect immunofluorescence were used for the localization of an esterase isoenzyme in human and monkey tissue culture cells and in monkey kidney frozen sections. In cultured cells, the esterase was found to be cytoplasmic in close apposition to the nucleus with a distribution resembling that of the Golgi region. The esterase in monkey kidney was predominantly found in the cytoplasm of the proximal convoluted tubules. There was slight staining in the Bowman's capsule of the glomerulus and in the collecting tubules of the medulla. It is suggested that the kidney is actually producing the esterase in its proximal tubule cells.