A NEW HISTOCHEMICAL METHOD FOR ACID PHOSPHATASE BY THE USE OF 5-IODOINDOXYL PHOSPHATE-Reference-Cited by-同舟云学术

A NEW HISTOCHEMICAL METHOD FOR ACID PHOSPHATASE BY THE USE OF 5-IODOINDOXYL PHOSPHATE

Published:1966-02 Issue:2 Volume:14 Page:171-176
ISSN:0022-1554
Container-title:Journal of Histochemistry & Cytochemistry
language:en
Short-container-title:J Histochem Cytochem.

Author:

EVANS G. W.¹,WHINNEY CECILIA L.¹,TSOU K. C.¹

Affiliation:

1. Harrison Department of Surgical Research, School of Medicine, University of Pennsylvania and The Laboratory of Surgical Pathology, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania

Abstract

5-Iodoindoxyl phosphate has been found to be a useful indigogenic substrate in the histochemical demonstration of acid phosphatase activity. Its superiority to other indoxyl phosphates is apparently due to a rapid oxidation of 5-iodoindoxyl to 5,5'-diiodoindigo in the acid pH range. A redox system of ferri-ferrocyanide enhances the oxidation and improves the localization. This method can be applied to calcium-formol-fixed tissues or to fresh frozen sections, although fixed tissues yield better results. The method is not recommended for the demonstration of enzyme activity in lipid-rich tissues because of the complexing property of lipids with 5,5'-diiodoindigo that results in crystal formation. The distribution of acid phosphatase activity with this method is generally similar to that obtained using azo dye methods.

Publisher

SAGE Publications

Subject

Histology,Anatomy

Link

http://journals.sagepub.com/doi/pdf/10.1177/14.2.171

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2. Modelluntersuchungen zur histochemischen Darstellung von Hydrolasen mit Tetrazoliumsalzen;Acta Histochemica;1988-01

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5. A new indigogenic method for the light and electron microscopic demonstration of 5?-nucleotide phosphodiesterases;The Histochemical Journal;1974-05