Purine nucleosidase (PNase) activity, probiotics potential, and food applicability of a newly-isolated <i>Levilactobacillus brevis</i> LAB42-Reference-Cited by-同舟云学术

Purine nucleosidase (PNase) activity, probiotics potential, and food applicability of a newly-isolated Levilactobacillus brevis LAB42

Published:2023-12-20 Issue: Volume: Page:
ISSN:1082-0132
Container-title:Food Science and Technology International
language:en
Short-container-title:Food sci. technol. int.

Author:

Ferdiansyah Mokhammad Khoiron¹^ORCID,Kang Hai-Seong²,Kim Ga Yeong¹,Park Beomseok³,Kularathna Ramesha M.R.E.³,Abraha Haftom Baraki¹,Kim Kwang-Pyo¹³

Affiliation:

1. Department of Food Science and Technology, College of Agriculture and Life Sciences, Jeonbuk National University, Jeonju, South Korea

2. Division of Food Microbiology, National Institute of Food and Drug Safety Evaluation, Cheongju, South Korea

3. Department of Agricultural Convergence Technology, College of Agriculture and Life Sciences, Jeonbuk National University, Jeonju, South Korea

Abstract

Hyperuricemia, a condition characterized by elevated levels of uric acid in the blood, is known as a risk factor for gout disease. In this study, we isolated a total of 72 MRS-grown colonies and evaluated their purine nucleosidase (PNase) activity. Among the isolated bacteria, Levilactobacillus (L.) brevis LAB42 displayed the highest PNase activity. Our findings also indicate that PNase activity can vary among lactic acid bacterial strains and during different growth phases. Based on the kinetics study, LAB42 consistently exhibits the highest PNase activity. Due to its ability to attach to Caco-2 cells and its resistance to acidic environments and bile exposure, L. brevis LAB42 was chosen for further studies and showed that with the right combination of additives, it has the potential to be an appropriate starter for milk fermentation.

Publisher

SAGE Publications

Subject

Industrial and Manufacturing Engineering,General Chemical Engineering,Food Science

Link

http://journals.sagepub.com/doi/pdf/10.1177/10820132231219859

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