HISTOCHEMICAL ALDEHYDE BLOCKADE BY ANILINE IN GLACIAL ACETIC ACID

Author:

LILLIE R. D.1,GLENNER G. G.1

Affiliation:

1. Laboratory of Pathology and Histochemistry, NIAMD, National Institutes of Health, Bethesda 14, Maryland

Abstract

More rapid and more complete blockade of tissue aldehydes produced by hydrochloric, periodic or peracetic acid may be achieved by use of molar solutions of aniline in glacial acetic acid than by use of molar aniline hydrochloride in water. A solution of 1% benzidine or o-dianisidine in glacial acetic acid is more effective than the above aniline solution against aldehydes produced by peracetic acid from lipids, but less effective against aldehydes produced by periodic acid from carbohydrates. Inclusion of 25 parts concentrated hydrochloric acid to 75 parts glacial acetic acid in the solvent impairs or inhibits the azomethine reaction of aniline with tissue aldehydes. A 20 to 30 minute exposure to a 10% volume dilution of aniline in glacial acetic acid should be adequate for testing the aldehyde nature of Schiff reactive loci in tissue, and in most instances failure of a 3 hour treatment to impair or prevent the following Schiff reaction can be taken to exclude aldehyde. The greater relative effect of o-dianisidine and benzidine in preventing the Schiff reaction of periodic or peracetic acid oxidized lipoids is of interest and merits further exploration. The colors produced by the o-dianisidine condensation with tissue aldehydes are disappointingly pale and this reaction would not appear to have any great histologic value.

Publisher

SAGE Publications

Subject

Histology,Anatomy

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