Modeling the Measurements of Cochlear Microcirculation and Hearing Function after Loud Noise

Author:

Arpornchayanon Warangkana12,Canis Martin34,Suckfuell Markus5,Ihler Fritz13,Olzowy Bernhard5,Strieth Sebastian16

Affiliation:

1. Walter Brendel Center for Experimental Medicine (WBex), University of Munich (LMU), Munich, Germany

2. Department of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

3. IFBLMU - Integrated Center for Research and Treatment of Vertigo, Balance and Ocular Motor Disorders, University of Munich (LMU), Munich, Germany

4. Department of Otorhinolaryngology, University of Goettingen, Goettingen, Germany

5. Department of Otorhinolaryngology, University of Munich (LMU), Munich, Germany

6. Department of Otorhinolaryngology, J. W. Goethe-University, Frankfurt/ Main, Germany

Abstract

Objective. Recent findings support the crucial role of microcirculatory disturbance and ischemia for hearing impairment especially after noise-induced hearing loss (NIHL). The aim of this study was to establish an animal model for in vivo analysis of cochlear microcirculation and hearing function after a loud noise to allow precise measurements of both parameters in vivo. Study Design. Randomized controlled trial. Setting. Animal study. Subjects and Methods. After assessment of normacusis (0 minutes) using evoked auditory brainstem responses (ABRs), noise (106-dB sound pressure level [SPL]) was applied to both ears in 6 guinea pigs for 30 minutes while unexposed animals served as controls. In vivo fluorescence microscopy of the stria vascularis capillaries was performed after surgical exposure of 1 cochlea. ABR measurements were derived from the contralateral ear. Results. After noise exposure, red blood cell velocity was reduced significantly by 24.3% (120 minutes) and further decreased to 44.5% at the end of the observation (210 minutes) in contrast to stable control measurements. Vessel diameters were not affected in both groups. A gradual decrease of segmental blood flow became significant (38.1%) after 150 minutes compared with controls. Hearing thresholds shifted significantly from 20.0 ± 5.5 dB SPL (0 minutes) to 32.5 ± 4.2 dB SPL (60 minutes) only in animals exposed to loud noise. Conclusion. With regard to novel treatments targeting the stria vascularis in NIHL, this standardized model allows us to analyze in detail cochlear microcirculation and hearing function in vivo.

Publisher

SAGE Publications

Subject

Otorhinolaryngology,Surgery

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3