ULTRASTRUCTURAL LOCALIZATION OF CONTRACTILE PROTEIN (THROMBOSTHENIN) IN HUMAN PLATELETS USING AN UNLABELED ANTIBODY-PEROXIDASE STAINING TECHNIQUE-Reference-Cited by-同舟云学术

ULTRASTRUCTURAL LOCALIZATION OF CONTRACTILE PROTEIN (THROMBOSTHENIN) IN HUMAN PLATELETS USING AN UNLABELED ANTIBODY-PEROXIDASE STAINING TECHNIQUE

Published:1971-09 Issue:9 Volume:19 Page:540-550
ISSN:0022-1554
Container-title:Journal of Histochemistry & Cytochemistry
language:en
Short-container-title:J Histochem Cytochem.

Author:

BOOYSE FRANCOIS M.¹²,ZSCHOCKE DOROTHEA¹²,RAFELSON MAX E.¹²,STERNBERGER LUDWIG A.¹²

Affiliation:

1. Department of Biochemistry, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612 and Department of Microbiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

2. Basic Sciences Department, Medical Research Laboratory, Edgewood Arsenal, Maryland 21010 and Department of Microbiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

Abstract

Soluble horseradish peroxidase-antihorseradish peroxidase (rabbit) complex was used to localize the actomyosin-like contractile protein, thrombosthenin, in both intact human platelets (Epon-embedded) and ultrathin sections (methacrylate-embedded). Antibody staining of ultrathin sections showed the presence of membrane-associated and cytoplasmic thrombosthenin. Antibody staining of intact cells showed that membrane-associated thrombosthenin was localized, at least in part, in the exterior, "fluffy" coat of the platelet. Even after prolonged fixation and/or incubation with the various antisera, we were unable to demonstrate antibody penetration of intact, fixed platelets. Brief treatment with Pronase removed the surface-localized proteinaceous material and completely abolished all antibody staining.

Publisher

SAGE Publications

Subject

Histology,Anatomy

Link

http://journals.sagepub.com/doi/pdf/10.1177/19.9.540

Cited by 45 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Redox Control of Platelet Function;Antioxidants & Redox Signaling;2009-05

2. Thiols in the IIb3 integrin are necessary for platelet aggregation;British Journal of Haematology;2008-08

3. Binding of Adenosine Diphosphate by Human Platelet Membrane;Novartis Foundation Symposia;2008-05-30

4. Myosin XVA Expression in the Pituitary and in Other Neuroendocrine Tissues and Tumors;The American Journal of Pathology;2001-10

5. Immunocytochemical studies on immunoglobulin-containing cells in the epidermis of rainbow trout Salmo gairdneri Richardson: influence of bath vaccination*;Journal of Fish Biology;1988-06