CYTOCHEMICAL DETECTION OF HYDROLASES IN FUNGUS CELLS III. ARYL SULFATASE

Abstract

In the cells of Aspergillus oryzae, Aspergillus nidulans, Neurospora crassa and Saccharomyces cerevisiae, aryl sulfatase can be demonstrated by incubation in a medium containing 6-bromo-2-naphthylsulfate as substrate and fast garnet GBC as coupling agent. Controls confirm the specificity of the reaction. Other incubation solutions (two Gomori media and a simultaneous coupling procedure with 8-hydroxyquinoline sulfate as substrate) gave negative results or reaction pictures equally to those in substrate-free control. The possible reasons for this are discussed. In the mycelial fungi the strongest enzyme activity is located in the most intensely metabolizing parts: tips of the hyphae and the differentiating parts of the conidiophores. The reaction granules in all four fungi are possibly identical with lysosomes.