DIHYDROLIPOIC DEHYDROGENASE ACTIVITY: A STEP IN FORMATION OF ACYL-COENZYME A, DEMONSTRATED HISTOCHEMICALLY-Reference-Cited by-同舟云学术

DIHYDROLIPOIC DEHYDROGENASE ACTIVITY: A STEP IN FORMATION OF ACYL-COENZYME A, DEMONSTRATED HISTOCHEMICALLY

Published:1964-06 Issue:6 Volume:12 Page:404-412
ISSN:0022-1554
Container-title:Journal of Histochemistry & Cytochemistry
language:en
Short-container-title:J Histochem Cytochem.

Author:

BALOGH KÁROLY¹

Affiliation:

1. Department of Pathology, Harvard Medical School, The James Homer Wright Pathology Laboratories, Massachusetts General Hospital, and the Mosher Pathology Laboratory, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts

Abstract

A method for the histochemical localization of dihydrolipoic dehydrogenase has been developed, using diphospyridine nucleotide (DPN) as a coenzyme and Nitro-blue tetrazolium (Nitro-BT) as the final electron acceptor. With this technique the enzyme, which plays an important role in the synthesis of coenzyme-A linked acyls, was found to be widespread in the tissues of the rat, and its distribution in various organs is presented. The problems involved in the histochemical demonstration of dihydrolipoic dehydrogenase are discussed.

Publisher

SAGE Publications

Subject

Histology,Anatomy

Link

http://journals.sagepub.com/doi/pdf/10.1177/12.6.404

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