Affiliation:
1. Medical Automation Research Center, University of Virginia Medical Center, Department of Pathology, Box 214, Charlottesville, VA 22908 USA
Abstract
Robotic pipetting stations are becoming increasingly popular to perform a variety of different laboratory analyses, including use of biochemical, chemical, and molecular biology reactions. As molecular diagnostic (MDx) analyses are adapted to pipetting stations, their need for additional resources on the pipetting stations such as heating and cooling will also grow. We designed and constructed small (< 150 cm2) accessories for use on a popular pipetting station. Both have small footprints and were built using readily available components. The microplate heater could achieve set point temperature within 10 minutes of activation and exhibited minimal temperature variation. The cooler was designed to be a passively cooled resource that could contain up to 15 Eppendorf tubes and maintain the temperature at least 20 degrees below ambient for at least two hours. Both units have been used to automate a coupled series of molecular biology reactions that yield RNA aptamers. We believe these two devices can be widely utilized with minor modifications of any pipetting station that requires heating or cooling capabilities on the pipetting station's deck.
Subject
Medical Laboratory Technology,Computer Science Applications
Cited by
1 articles.
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