Affiliation:
1. Department of Otolaryngology—Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, China
2. Otolaryngological Institute, Shanghai Jiao Tong University, Shanghai, China
3. Shanghai Key Laboratory of Sleep Disordered Breathing, Shanghai, China
Abstract
Introduction: SKF-96365 is regarded as an inhibitor of receptor-mediated calcium ion (Ca2+) entry. The current study aimed to explore the effects of SKF-96365 on murine allergic rhinitis (AR). Methods: Intranasal SKF-96365 administration was performed on OVA induced murine AR. Serum and nasal lavage fluid (NLF) from mice were harvested to assay IgE and inflammatory cytokines using ELISA method. Inflammatory cells were counted and analyzed in NLF. Nasal mucosa tissues were collected from mice and used for HE staining, immunohistochemistry (IHC) staining, and real-time PCR detection. Results: SKF-96365 had therapeutic effects on murine AR manifesting attenuation of sneezing, nasal rubbing, IgE, inflammatory cytokines, inflammatory cells, TRPC6 immunolabeling, and TRPC6, STIM1 and Orai1 mRNA levels in AR mice. Conclusion: SKF-96365 could effectively alleviate the symptoms of murine AR. SKF-96365 could suppress TRPC6, STIM1, and Orai1 activities, leading to the downregulation of inflammatory cytokines and inflammatory cells in murine AR.
Funder
science and technology commission of shanghai municipality
science and technology innovation plan of shanghai science and technology commission
National Natural Science Foundation of China
shanghai shenkang hospital development center
Natural Science Foundation of Shanghai
Subject
Pharmacology,Immunology,Immunology and Allergy
Cited by
3 articles.
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