Effect of short peptides on neuronal differentiation of stem cells

Author:

Caputi Sergio1,Trubiani Oriana1ORCID,Sinjari Bruna1,Trofimova Svetlana2,Diomede Francesca1,Linkova Natalia23,Diatlova Anastasia23,Khavinson Vladimir24

Affiliation:

1. Laboratory of Stem Cells and Regenerative Medicine, Department of Medical, Oral and Biotechnological Sciences, School of Medicine and Health Sciences, University “G. d’Annunzio” Chieti-Pescara, Chieti, Italy

2. Department of Biogerontology, Saint Petersburg Institute of Bioregulation and Gerontology, Saint Petersburg, Russia

3. Department of Medical Physic, Peter the Great St. Petersburg Polytechnic University, Saint Petersburg, Russia

4. Group of Peptide Regulation of Ageing, Pavlov Institute of Physiology of RAS, Saint Petersburg, Russia

Abstract

It has been demonstrated that short peptides play an important role in the transmission of biological information, modulation of transcription, and restoring genetically conditioned alterations occurring with age. Peptidergic regulation of homeostasis occupies an important place in physiological processes, which lead to the aging of cells, tissues, and organs, consisting in the involution of major regulatory systems—the nervous, the endocrine, and the immune. The effect of AED (Ala-Glu-Asp), KED (Lys-Glu-Asp), KE (Lys-Glu), AEDG (Ala-Glu-Asp-Gly) peptides and their compound on neuronal differentiation of human periodontal ligament stem cells (hPDLSCs) was studied by immunofluorescence and western blot analysis. Growth-Associated Protein 43 (GAP43), which implements neurotransmission mechanisms and neuroplasticity, demonstrated an increased expression in hPDLSCs cultured with a compound of all studied peptides and with KED alone. The peptide compound and KED, increase the expression of Nestin (neurofilament protein), expressed in early neuronal precursors in hPDLSCs cultures. Thus, the compound of peptides AEDG, KE, AED, and KED could promote the neuronal differentiation of hPDLSCs and be a promising tool for the study of peptides as a modulator of neurogenesis in neurodegenerative diseases studied in animal models.

Publisher

SAGE Publications

Subject

Pharmacology,Immunology,Immunology and Allergy

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