Platelet-Derived Growth Factor Receptor Expression after Neural Grafting in a Rat Model of Parkinson's Disease

Author:

Ballagi Andrea E.1,Odin Per2,Othberg-Cederström Agneta2,Smits Anja13,Duandr Wei-Ming2,Lindvall Olle2,Funa Keiko1

Affiliation:

1. Ludwig Institute for Cancer Research, Biomedical Center, S-751 24 Uppsala, Sweden

2. Department of Neurology, Restorative Neurology Unit, University Hospital, S-221 85 Lund, Sweden

3. Department of Neurology, University Hospital, S-751 85 Uppsala, Sweden

Abstract

Platelet-derived growth factor (PDGF) has trophic effect on dopaminergic neurons in vitro. We have previously shown dynamic changes in the expression of PDGF in embryonic mesencephalic grafts and surrounding host striatal tissue following intracerebral transplantation in a rat model of Parkinson's disease. In this study the expression of the PDGF receptors was examined in the same model using immunohistochemistry. Most ventral mesencephalic (VM) cells from E13–E15 rat embryos possessed both PDGF α-and β-receptors before implantation. Double immunofluorescence staining revealed that about 10% of the cells also expressed tyrosine hydroxylase (TH). The PDGF α-receptor was detectable in the graft up to 1 wk after transplantation but had disappeared at 3 wk. In the host tissue, scattered glial cells were positive for the α-receptor but the expression was unchanged following transplantation. The β-receptor expression almost completely disappeared from the grafted tissue by 4 h following transplantation, and only a few cells of the host striatum showed immunoreactivity. However, after 3 wk β-receptor positive cells were again detectable in the graft. These cells appeared to be endothelial cells as identified by an antibody against von Willebrand's factor. Our data suggest that PDGF might act locally on embryonic dopaminergic cells in an autocrine or juxtacrine manner before and shortly after transplantation, and on surrounding glial cells in a paracrine manner after transplantation. Furthermore, PDGF-BB might influence neovascularization in the graft.

Publisher

SAGE Publications

Subject

Transplantation,Cell Biology,Biomedical Engineering

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