Affiliation:
1. Tampa Woman's Health Center, 2010 E. Fletcher Avenue, Tampa, FL 33612, USA
2. Department of Pharmacology and Experimental Therapeutics, Division of Neurosurgery, University of South Florida, 4 Columbia Drive, Suite 730, Tampa, FL 33606, USA
Abstract
Successful transplantation of cadaver embryonic neural tissue is highly dependent on the method used to obtain the tissue. It is important that the tissue not be contaminated bacteriologically by vaginal flora during the procedure, and that it not be disrupted mechanically. A low-pressure aspiration abortion technique has been developed that allows for the safe, effective obtainment of embryonic tissue and reduces the risk of transmitting infection. Tissue was cultured in vitro in 102 cases with minimal evidence of contamination by vaginal flora. Transplants of neural tissue into over 300 rodents have resulted in no intracranial abscesses, even in the setting of immunosuppression. The suction apparatus and low-pressure aspiration minimize disruption of the embryonic tissue. Use of low pressure adds no significant additional risk to the patient. In over 300 cases, there have been no medical complications specifically attributable to the technique. Because local anesthetic is used and sonography is not routinely required, the procedure can easily be performed in an outpatient setting during routine elective abortions, with minimal slowing or disruption of the clinic's surgical schedule. In conclusion, the low-pressure aspiration abortion technique can be safely and effectively used to obtain embryonic and early gestational fetal tissue that is almost always free from bacterial, fungal, and yeast contamination and that is frequently structurally intact. It requires no significant alteration in indications for abortion, risks, methodology, timing of the abortion, or patient management.
Subject
Transplantation,Cell Biology,Biomedical Engineering
Cited by
31 articles.
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