Development of a Novel Pre-Vascularized Three-Dimensional Skin Substitute Using Blood Plasma Gel

Author:

Dai Niann-Tzyy1ORCID,Huang Wen-Shyan2,Chang Fang-Wei3,Wei Lin-Gwei4,Huang Tai-Chun1,Li Jhen-Kai1,Fu Keng-Yen1,Dai Lien-Guo5,Hsieh Pai-Shan1,Huang Nien-Chi1,Wang Yi-Wen6,Chang Hsin-I7,Parungao Roxanne8,Wang Yiwei8

Affiliation:

1. Division of Plastic and Reconstructive Surgery, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, R.O.C.

2. Plastic and Reconstructive Surgery, Zouying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan, R.O.C.

3. Department of Obstetrics & Gynecology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, R.O.C.

4. Division of Plastic and Reconstructive Surgery, Taoyuan Armed Forces General Hospital, Taoyuan, Taiwan, R.O.C.

5. Department of Orthopedics, Shuang Ho Hospital, Taipei Medical University, New Taipei, Taiwan, R.O.C.

6. Department of Biology and Anatomy, National Defense Medical Center, Taipei, Taiwan, R.O.C.

7. Department of Biochemical Science and Technology, National Chiayi University, Chiayi, Taiwan, R.O.C.

8. Burns Research Group, ANZAC Research Institute, Concord Hospital, University of Sydney, New South Wales, Australia

Abstract

Skin substitutes with existing vascularization are in great demand for the repair of full-thickness skin defects. In the present study, we hypothesized that a pre-vascularized skin substitute can potentially promote wound healing. Novel three-dimensional (3D) skin substitutes were prepared by seeding a mixture of human endothelial progenitor cells (EPCs) and fibroblasts into a human plasma/calcium chloride formed gel scaffold, and seeding keratinocytes onto the surface of the plasma gel. The capacity of the EPCs to differentiate into a vascular-like tubular structure was evaluated using immunohistochemistry analysis and WST-8 assay. Experimental studies in mouse full-thickness skin wound models showed that the pre-vascularized gel scaffold significantly accelerated wound healing 7 days after surgery, and resembled normal skin structures after 14 days post-surgery. Histological analysis revealed that pre-vascularized gel scaffolds were well integrated in the host skin, resulting in the vascularization of both the epidermis and dermis in the wound area. Moreover, mechanical strength analysis demonstrated that the healed wound following the implantation of the pre-vascularized gel scaffolds exhibited good tensile strength. Taken together, this novel pre-vascularized human plasma gel scaffold has great potential in skin tissue engineering.

Funder

Ministry of Science and Technology, ROC

Publisher

SAGE Publications

Subject

Transplantation,Cell Biology,Biomedical Engineering

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