Transforming growth factor-β3 expression up-regulates on cleft palates induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice

Author:

Gan Li-qiang1,Fu Yue-xian1,Liu Xing1,Qiu Lin1,Wu Sheng-de1,Tian Xiao-fei1,Liu Yan1,Wei Guang-hui2

Affiliation:

1. Department of Pediatric Surgery, Chongqing Children’s Hospital, Chongqing Medical University, Chongqing, China

2. Department of Pediatric Surgery, Chongqing Children’s Hospital, Chongqing Medical University, Chongqing, China,

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) has been shown to induce cleft palate, in which the molecular etiology of the defect is poorly characterized. Recently, transforming growth factor-β3 (TGF-β3) has been indicated to play an essential role in the development of palatal shelves. In this developmental toxicity study, we investigated the effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the expression of TGF-β3 in fetal mice. Pregnant C57BL/6 mice were exposed to corn oil or TCDD (32 μg/kg/day 64 μg/kg/day, per os) at embryonic day 10 (ED10), a drastic inhibition of palatal shelves was induced. By using RT-PCR (reverse transcription-polymerase chain reaction) and Western blot, the expressions of TGF-β3 was investigated. We found that the expression of TGF-β3 was gradually up-regulated in TCDD-treated group. These results suggest that cleft palate can be induced by TCDD exposure, the modification of TGF-β3 is related to its pathogenesis.

Publisher

SAGE Publications

Subject

Health, Toxicology and Mutagenesis,Public Health, Environmental and Occupational Health,Toxicology

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