Evaluation of Thrombin Generation Assay in Patients With Hemophilia

Abstract

We evaluated the correlation between thrombin generation (TG) parameters with bleeding symptoms and disease severity in patients with hemophilia. In this cross-sectional study, 59 patients with hemophilia without inhibitors and regardless of their severity were randomly selected from southern Iran and TG assays were conducted. Bleeding score (BS) was calculated by performing a clinical evaluation using Tosetto questionnaire. Only lag time showed a statistically significant correlation with BS ( rs = .316, P = .016). All TG parameters except peak showed association with disease severity ( P < .05). Endogenous thrombin potential showed a significant correlation with factor activity level ( rs = .459, P < .001). Both lag time and start tail showed significant negative correlations with factor activity level ( rs = −0.488, P < .001 and rs= − .289, P < .026, respectively). Although most of the TG parameters evaluated were not significantly correlated with the BS of patients with hemophilia, the majority of TG parameters were significantly associated with factor activity level and disease severity.