Comparison of two prevalent individually ventilated caging systems for detection of murine infectious agents via exhaust air particles-Reference-Cited by-同舟云学术

Comparison of two prevalent individually ventilated caging systems for detection of murine infectious agents via exhaust air particles

Published:2018-07-17 Issue:1 Volume:53 Page:84-88
ISSN:0023-6772
Container-title:Laboratory Animals
language:en
Short-container-title:Lab Anim

Author:

Mahabir Esther¹^ORCID,Durand Stephanie²,Henderson Kenneth S²,Hardy Patrick³

Affiliation:

1. Comparative Medicine, Center for Molecular Medicine, University of Cologne, Cologne, Germany

2. Charles River Laboratories, Wilmington, USA

3. Allentown Inc., Veterinary and Professional Services, Allentown, USA

Abstract

We compared the efficacies of two different individually ventilated cage systems (Allentown and Tecniplast) for health monitoring (HM) of murine infectious agents using exhaust air particle (EAP) capture and real-time PCR. After three months of monitoring, both EAP capture media allowed detection of Helicobacter, Pasteurella and Entamoeba. Use of the EAP real-time PCR for HM reduces the number of mice used.

Publisher

SAGE Publications

Subject

General Veterinary,Animal Science and Zoology

Link

http://journals.sagepub.com/doi/pdf/10.1177/0023677218785929

Reference8 articles.

1. Microbiological monitoring of laboratory mice and biocontainment in individually ventilated cages: a field study

2. Murine norovirus detection in the exhaust air of IVCs is more sensitive than serological analysis of soiled bedding sentinels

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