Affiliation:
1. INSERM U442, IFR-FR 46, Université Paris Sud, Orsay, France
2. INSERM U469 Université Montpellier I, Montpellier, France
3. Service d'lmagerie Cellulaire, Université Paris Sud, Orsay, France
Abstract
In freshly isolated rat hepatocyte multiplets, Ca2+ signals in response to vasopressin are highly organized. In this study we used specific probes to visualize, by fluorescence and confocal microscopy, the main signaling molecules involved in vasopressin-mediated Ca2+ responses. V1a receptors were detected with a novel fluorescent antagonist, Rhm8 -PVA. The Gαq/Gα11, PLCβ3, PIP2, and InsP3 receptors were detected with specific antibodies. V1a vasopressin receptors and PIP2 were associated with the basolateral membrane and were not detected in the bile canalicular domain. Gαq/Gα11, PLCβ3, and InsP3 receptors were associated with the basolateral membrane and also with other intracellular structures. We used double labeling, Western blotting, and drugs (cytochalasin D, colchicine) known to disorganize the cytoskeleton to demonstrate the partial co-localization of Gαq/Gα11 with F-actin.
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17 articles.
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