Nuclear Detection of Cellular Retinoic Acid Binding Proteins I and II with New Antibodies

Author:

Gaub Marie-Pierre1,Lutz Yves1,Ghyselinck Norbert B.1,Scheuer Isabelle1,Pfister Véronique1,Chambon Pierre1,Rochette-Egly Cécile1

Affiliation:

1. Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP/Collège de France, France

Abstract

Apart from the retinoic acid nuclear receptor family, there are two low molecular weight (15 kD) cellular retinoic acid binding proteins, named CRABPI and II. Mouse monoclonal and rabbit polyclonal antibodies were raised against these proteins by using as antigens either synthetic peptides corresponding to amino acid sequences unique to CRABPI or CRABPII, or purified CRABP proteins expressed in E. coli. Antibodies specific for mouse and/or human CRABPI and CRABPII were obtained and characterized by immunocytochemistry and immunoblotting. They allowed the detection not only of CRABPI but also of CRABPII in both nuclear and cytosolic extracts from transfected COS-1 cells, mouse embryos, and various cell lines.

Publisher

SAGE Publications

Subject

Histology,Anatomy

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