Protease XXIV Increases Detection of Mucin Gene Expression during In Situ Hybridization in Archival Tissue-Reference-Cited by-同舟云学术

Protease XXIV Increases Detection of Mucin Gene Expression during In Situ Hybridization in Archival Tissue

Published:2001-07 Issue:7 Volume:49 Page:923-924
ISSN:0022-1554
Container-title:Journal of Histochemistry & Cytochemistry
language:en
Short-container-title:J Histochem Cytochem.

Author:

Rheinhardt Jeanette M.¹,Finkbeiner Walter E.¹

Affiliation:

1. Department of Pathology, University of California–Davis, Davis, California

Abstract

Digoxigenin-labeled riboprobes of six groups of human mucins were evaluated for sensitivity in archival tissue, using protease XXIV or proteinase K during in situ hybridization.

Publisher

SAGE Publications

Subject

Histology,Anatomy

Link

http://journals.sagepub.com/doi/pdf/10.1177/002215540104900714

Reference8 articles.

1. Alkaline fixation drastically improves the signal of in situ hybridization

2. Non-isotopic in situ detection of mRNA for interleukin-4 in archival human tissue

3. Optimization of non-isotopicin situ hybridization on formalin-fixed, paraffin-embedded material using digoxigenin-labelled probes and transgenic tissues

4. HBV-DNA by in situ hybridization. A method to improve sensitivity on formalin-fixed, paraffinembedded liver biopsies

5. In situ hybridisation in formalin fixed, paraffin wax embedded liver specimens: method for detecting human and viral DNA using biotinylated probes.

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