Lac z Histochemistry and Immunohistochemistry Reveal Ephrin-B Ligand Expression in the Inner Ear-Reference-Cited by-同舟云学术

Lac z Histochemistry and Immunohistochemistry Reveal Ephrin-B Ligand Expression in the Inner Ear

Published:2002-12 Issue:12 Volume:50 Page:1641-1645
ISSN:0022-1554
Container-title:Journal of Histochemistry & Cytochemistry
language:en
Short-container-title:J Histochem Cytochem.

Author:

Bianchi Lynne M.¹,Dinsio Kyl¹,Davoli Katherine¹,Gale Nicholas W.²

Affiliation:

1. Neuroscience Program, Oberlin College, Oberlin, Ohio

2. Regeneron Pharmaceuticals, Tarrytown, New York

Abstract

Immunostaining in transgenic mice carrying the lac z gene can be used to map gene and protein distribution in a single tissue. In this study, we examined inner ears from ephrin-B3 homozygous and ephrin-B2 heterozygous mice. Ephrin-B3 lac z expression was limited in these mice. However, immunostaining revealed ephrin-B3 throughout cochlear and vestibular regions. Immunoreactivity was absent in ephrin-B3-homozygous null mutants, demonstrating the specificity of the antibody. Ephrin-B2 lac z reactivity was detected in a limited number of cells in cochlear and vestibular regions. Different immunostaining patterns were found with different antibodies. Comparison with lac z expression indicated which antibody was specific for the transmembrane-bound ephrin-B2 ligand.

Publisher

SAGE Publications

Subject

Histology,Anatomy

Link

http://journals.sagepub.com/doi/pdf/10.1177/002215540205001208

Reference9 articles.

1. Degeneration of vestibular neurons in late embryogenesis of both heterozygous and homozygous BDNF null mutant mice

2. Distribution of Eph-related molecules in the developing and mature cochlea

3. Comparison of Ephrin-A ligand and EphA receptor distribution in the developing inner ear

4. EphB2 Guides Axons at the Midline and Is Necessary for Normal Vestibular Function

5. Ephrin-B2 Selectively Marks Arterial Vessels and Neovascularization Sites in the Adult, with Expression in Both Endothelial and Smooth-Muscle Cells

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