Quadruple Immunofluorescence: A Direct Visualization Method-Reference-Cited by-同舟云学术

Quadruple Immunofluorescence: A Direct Visualization Method

Published:1997-02 Issue:2 Volume:45 Page:155-158
ISSN:0022-1554
Container-title:Journal of Histochemistry & Cytochemistry
language:en
Short-container-title:J Histochem Cytochem.

Author:

Ferri Gian-Luca¹,Gaudio Rosa M.¹,Castello Ida F.¹,Berger Peter²,Giro Gabriele³

Affiliation:

1. Neuro- and Endocrine Research, Department of Cytomorphology, University of Cagliari, and “Oasi” IRCCS, Troina, Italy

2. Institute for Biomedical Aging Research, Austrian Academy of Sciences, Innsbruck, Austria

3. FRAE Institute, CNR, Bologna, Italy

Abstract

We describe fluorescence immunostaining of four different antigens in the same section. The fluorochrome conjugates used show highest emission in the blue, green, yellow, and red regions of the visible light spectrum, respectively. Specially designed single fluorochrome filter combinations allow selective visualization of each fluorochrome label in turn, without visible crosstalk with the others.

Publisher

SAGE Publications

Subject

Histology,Anatomy

Link

http://journals.sagepub.com/doi/pdf/10.1177/002215549704500201

Reference12 articles.

1. Antigenic Features of Human Protein Hormones Elucidated by Monoclonal Antibodies

2. Chapter 4 Multicolor Laser Scanning Confocal Immunofluorescence Microscopy: Practical Application and Limitations

3. The "VGF" protein in rat adenohypophysis: sex differences and changes during the estrous cycle and after gonadectomy.

Cited by 28 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. TUNEL-n-DIFL Method for Detection and Estimation of Apoptosis Specifically in Neurons and Glial Cells in Mixed Culture and Animal Models of Central Nervous System Diseases and Injuries;Methods in Molecular Biology;2024

2. Advances in imaging and analysis of 4 fluorescent components through the rat cortical column;Journal of Neuroscience Methods;2020-07

3. Simultaneous Polychromatic Immunofluorescent Staining of Tissue Sections and Consecutive Imaging of up to Seven Parameters by Standard Confocal Microscopy;Current Protocols in Cytometry;2019-09-24

4. Fully automated 5-plex fluorescent immunohistochemistry with tyramide signal amplification and same species antibodies;Laboratory Investigation;2017-05-15

5. Garbage in, garbage out: A critical evaluation of strategies used for validation of immunohistochemical biomarkers;Molecular Oncology;2014-03-21