Simple and Rapid Methods for SEM Observation and TEM Immunolabeling of Rubber Particles-Reference-Cited by-同舟云学术

Simple and Rapid Methods for SEM Observation and TEM Immunolabeling of Rubber Particles

Published:2003-08 Issue:8 Volume:51 Page:1105-1108
ISSN:0022-1554
Container-title:Journal of Histochemistry & Cytochemistry
language:en
Short-container-title:J Histochem Cytochem.

Author:

Singh Adya P.¹,Wi Seung Gon²,Kang Hunseung¹,Chung Gap Chae¹,Kim Yoon Soo²

Affiliation:

1. Agricultural Plant Stress Research Center, Chonnam National University, Gwangju, Republic of Korea

2. Department of Forest Products and Technology, Chonnam National University, Gwangju, Republic of Korea

Abstract

We developed a method involving air-drying of a rubber suspension after fixation in glutaraldehyde-tannic acid and postfixation in osmium tetroxide for SEM observation. For TEM immunolabeling the suspension was air-dried after osmium-only fixation. Whereas conventional methods failed to satisfactorily stabilize rubber particles, the methods described here proved successful in preserving their integrity.

Publisher

SAGE Publications

Subject

Histology,Anatomy

Link

http://journals.sagepub.com/doi/pdf/10.1177/002215540305100815

Reference9 articles.

1. The Effect of Chemical Fixation and Dehydration on the Preservation of Latex inCalystegia silvatica(Convolvulaceae). Examination of Exudate and Latexin situby Light and Scanning Electron Microscopy

2. Rubber particles from four different species, examined by transmission electron microscopy and electron-paramagnetic-resonance spin labeling, are found to consist of a homogeneous rubber core enclosed by a contiguous, monolayer biomembrane

3. Immuno Electron Microscopic Localization of Peroxidases in the Differentiating Xylem of Populus spp.

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