Clinical Significance of Telomere Length and Associated Proteins in Oral Cancer

Author:

Sainger Rachana N.1,Telang Shaila D.2,Shukla Shilin N.3,Patel Prabhudas S.1

Affiliation:

1. Biochemistry Research Division, The Gujarat Cancer & Research Institute, Asarwa, Ahmedabad–380 016, India.

2. Department of Biochemistry, M.S. University of Baroda, Vadodara–390 001, India.

3. The Gujarat Cancer & Research Institute, Asarwa, Ahmedabad–380 016, India.

Abstract

Purpose Telomere shortening is an important event during carcinogenesis. Although studies suggest role of multiple proteins in telomere length regulation, there is dearth of reports in oral cancer which is a leading malignancy in Asian countries especially in India. Thus the present study was carried out to study these mechanisms and explore the pathways involved in telomere—telomerase regulation and identify possible prognostic markers to understand the biology of oral tumors for better treatment approaches. Methods Telomere length was determined by Southern Hybridisation method, telomeric repeat binding factor (TRF) 1 and 2 expression was detected by Western blot method and telomerase activation by telomeric repeat amplification protocol. Statistical analysis was done using SPSS (Version 10) software. Results Significant shortening of telomeres was seen in the tumor tissues as compared to normal tissues. Poor prognosis was observed in the patients with higher telomere length in malignant tissue, higher tumor to normal telomere length ratio (T/N TRF LR). Expression of TRF-2 but not TRF-1 protein was significantly higher in the malignant tissues. We also observed telomerase activation in 75 malignant tissues. Conclusions Our results reveal significant clinical usefulness of telomere length, T/N TRF LR and telomerase activation in the prognosis of oral cancer patients. TRF-2 overexpression in malignant tissues appears to play an important role in telomere length shortening in oral cancer.

Publisher

SAGE Publications

Subject

Biochemistry, medical,Pharmacology,Molecular Medicine

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