The synthesis and preliminary cytotoxicity evaluation of hexahydrodispiro[indole-3,3′-indolizine-2′,3″-piperidine]-2(1H),4″-dione compounds

Abstract

Seven hexahydrodispiro[indole-3,3′-indolizine-2′,3″-piperidine]-2(1 H),4″-dione compounds were synthesized by a catalyst-free 1,3-dipolar cycloaddition reaction in a one-pot three-component system containing 3,5-diarylidene-1-benzylpiperidin-4-one, isatin and l-pipecolic acid. The structures of these compounds were characterized by nuclear magnetic resonance, high-resolution mass spectrometry and X-ray data analysis. These chemical entities were evaluated for their cytotoxicity (brine shrimp assay), and five compounds were found potential in this bioassay. Compound 2e was the most prominent target against Artemia salina with an LC50 value of 7.27 μg/mL.