Rhubarb extract rebuilding the mucus homeostasis and regulating mucin-associated flora to relieve constipation

Author:

Gao Han12,He Chengwei1,Xin Shuzi1,Hua Rongxuan3,Du Yixuan4,Wang Boya5,Gong Fengrong3,Yu Xinyi3,Pan Luming3,Liang Chen3,Gao Lei6,Shang Hongwei7,Xu Jing-dong1ORCID

Affiliation:

1. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

2. Department of Clinical Laboratory, Aerospace Center Hospital, Beijing 100039, China

3. Department of Clinical Medicine, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

4. Department of Oral Medicine, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

5. Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Gastrointestinal Oncology, Peking University Cancer Hospital & Institute, Beijing 100142, China

6. Department of Biomedical Informatics, School of Biomedical Engineering, Capital Medical University, Beijing 100069, China

7. Experimental Center for Morphological Research Platform, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Abstract

In clinical trials, rhubarb extract (Rb) was demonstrated to efficiently alleviate constipation. We would like to find out the underlying mechanism of rhubarb relieving constipation. However, there are few studies on the effects of rhubarb on colonic mucus secretion and constipation. The aim of this study was to investigate the effects of rhubarb on colonic mucus secretion and its underlying mechanism. The mice were randomly divided into four groups. Group I was the control group and Group II was the rhubarb control group, with Rb (24 g/kg body weight [b.w.]) administered through intragastric administration for three days. Group III mice were given diphenoxylate (20 mg/kg b.w.) for five days via gavage to induce constipation. Group IV received diphenoxylate lasting five days before undergoing Rb administration for three days. The condition of the colon was evaluated using an endoscope. Particularly, the diameter of blood vessels in the colonic mucosa expanded considerably in constipation mice along with diminishing mucus output, which was in line with the observation via scanning electron microscope (SEM) and transmission electron microscope (TEM). We also performed metagenomic analysis to reveal the microbiome related to mucin gene expression level referring to mucin secretion. In conclusion, Rb relieves constipation by rebuilding mucus homeostasis and regulating the microbiome.

Funder

National Natural Science Foundation of China

Publisher

Frontiers Media SA

Subject

General Biochemistry, Genetics and Molecular Biology

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