Modulation of CD8+ Intraepithelial Lymphocyte Distribution by Dietary Fiber in the Rat Large Intestine1

Abstract

We studied whether ingestion of dietary fiber modifies the distribution of intraepithelial lymphocytes (IEL) in a physiological condition. Male WKAH rats were fed diets either with fiber (sugar beet fiber or crystalline cellulose, 100 g/kg diet each) or without fiber for 3 weeks. The number of CD8+, CD4+, and NKR-P1+ IEL per epithelial layer in the crypt section of the cecum, proximal colon, and distal colon were scored by immunohistochemical staining. We found that the proportion of CD8+ IEL was greater in the cecal mucosa and was gradually reduced toward the distal large intestine in general. In contrast, there was no difference in the proportion of CD4+ and NKR-P1+ IEL in the large intestine. Dietary sugar beet fiber, but not crystalline cellulose, increased the proportion of CD8+ IEL, especially in the cecal mucosa, but not the CD4+ and NKR-P1+ IEL. Analysis of cecal organic acid concentration confirmed higher concentrations of acetate and butyrate, and lower concentration of succinate and isovalerate, in the cecum of the rats fed sugar beet fiber than other diets. These results indicate that ingestion of some dietary fiber modulates local cell proliferation of a progenitor of CD8+ IEL or promotes homing of CD8+ T cells into the large intestinal epithelium, most likely via the fermentation in the luminal contents.