Age-associated expression of HCN channel isoforms in rat sinoatrial node-Reference-Cited by-同舟云学术

Age-associated expression of HCN channel isoforms in rat sinoatrial node

Published:2015-09-03 Issue:3 Volume:241 Page:331-339
ISSN:1535-3702
Container-title:Experimental Biology and Medicine
language:en
Short-container-title:Exp Biol Med (Maywood)

Author:

Huang Xin¹,Yang Pei²,Yang Zhao³,Zhang Hong⁴,Ma Aiqun¹

Affiliation:

1. Department of Cardiology, First Affiliated Hospital of Xi’an Jiaotong University Health Science Center, Ion Channel Disease Laboratory, Key Laboratory of Environment and Genes related to Diseases of Education Ministry, Xi’an, Shaanxi 710061, P.R. China

2. Department of Orthopedics, Second Affiliated Hospital of Xi’an Jiaotong University Health Science Center, Xi’an, Shaanxi 710004, P.R. China

3. Institute of Medical Electronics in Medical School, Key Laboratory of Biomedical Information Engineering, Ministry of Education, Xi’an Jiaotong University, Xi’an, Shaanxi 710061, P.R. China

4. School of Electrical Engineering, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, P.R. China

Abstract

The expression of hyperpolarization-activated cyclic nucleotide-gated cation (HCN) channel isoforms varies among species, cardiac tissues, developmental stages, and disease generation. However, alterations in the HCN channels during aging remain unclear. We investigated the protein expressions of HCN channel isoforms, HCN1-HCN4, in the sinoatrial nodes (SANs) from young (1-month-old), adult (4-month-old), and aged (30-month-old) rats. We found that HCN2 and HCN4 proteins were present in rat SAN using immunohistochemistry; therefore, we quantitatively analyzed their expression by Western blot. Aim to correlate protein expression and pacemaking function, specific blockade of HCN channels with 3 µmol/L ivabradine prolonged the cycle length in the intact rat heart. During the senescent process, the HCN2 and HCN4 protein levels declined, which was accompanied with a decreased effect of ivabradine on rat SAN automaticity. These results indicated the age-associated expression and relative function of HCN channel isoforms.

Publisher

SAGE Publications

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://journals.sagepub.com/doi/pdf/10.1177/1535370215603515

Reference39 articles.

1. MANAGEMENT OF ATRIAL FIBRILLATION, VENTRICULAR ARRHYTHMIAS AND PACEMAKERS IN OLDER PERSONS: Permanent Pacemakers in Older Persons

2. Electrophysiology of the ageing rabbit and cat sinoatrial node -- a comparative study

Cited by 22 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Voltage-gated ion channel’s gene expression in the myocardium of embryo and adult chickens;Developmental Biology;2024-12

2. Dopaminergic amacrine cells express HCN channels in the developing and adult mouse retina;2024-07-23

3. An anchor-tether ‘hindered’ HCN1 inhibitor is antihyperalgesic in a rat spared nerve injury neuropathic pain model;British Journal of Anaesthesia;2023-10

4. Sinus node dysfunction and atrial fibrillation—Relationships, clinical phenotypes, new mechanisms, and treatment approaches;Ageing Research Reviews;2023-04

5. Depressed HCN4 function in the type 2 diabetic sinoatrial node;Molecular and Cellular Biochemistry;2022-12-27