The lipid A of Burkholderia multivorans C1576 smooth-type lipopolysaccharide and its pro-inflammatory activity in a cystic fibrosis airways model

Author:

Ieranò Teresa1,Cescutti Paola2,Leone Maria Rosaria1,Luciani Alessandro3,Rizzo Roberto2,Raia Valeria4,Lanzetta Rosa1,Parrilli Michelangelo1,Maiuri Luigi5,Silipo Alba1,Molinaro Antonio6

Affiliation:

1. Dipartimento di Chimica Organica e Biochimica, Università degli Studi di Napoli Federico , Napoli, Italy

2. Dipartimento di Scienze della Vita, Università degli Studi di Trieste, Trieste, Italy

3. Institute of Pediatrics, University of Foggia and Dynamic Imaging Microscopy, CEINGE, Naples, Italy

4. Department of Pediatrics, Università degli Studi di Napoli Federico , Naples, Italy

5. Institute of Pediatrics, University of Foggia and European Institute for Research in Cystic Fibrosis San Raffaele Scientific Institute, Milan, Italy

6. Dipartimento di Chimica Organica e Biochimica, Università degli Studi di Napoli Federico , Napoli, Italy,

Abstract

Cystic fibrosis is an autosomal recessive disorder and it is characterised by chronic bacterial airway infection which leads to progressive lung deterioration, sometimes with fatal outcome. Burkholderia multivorans and Burkholderia cenocepacia are the species responsible for most of the infections of cystic fibrosis patients. Lipopolysaccharide endotoxins (LPSs) are among the foremost factors of pathogenesis of Gram-negative infection and, in particular, lipid A is the endotoxic portion of LPS responsible for eliciting host innate immune response. In this work, the complete primary structure of the lipid A from B. multivorans C1576 has been defined and, further, its pro-inflammatory activity in a cystic fibrosis airways model is shown. The structure of B. multivorans lipid A was attained by chemical, mass spectrometry and nuclear magnetic resonance analyses whereas its biological activity was assessed on the intestinal epithelial cell line CACO-2 cells, on the airway epithelial IB3-1 cells, carrying the ΔF508/W1282X CFTR mutation and on an ex vivo model of culture explants of nasal polyps.

Publisher

SAGE Publications

Subject

Infectious Diseases,Cell Biology,Molecular Biology,Immunology,Microbiology

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