Abstract
A new procedure is reported for the staining of DNA, for flow microfluorimetry. It allows the production of stained cell nuclei in a single step by incorporating the DNA stain with a solution of the nonionic detergent Triton-X-100. This method has been found to be applicable to all DNA fluorochromes tested (ethidium bromide, propidium iodide, mithramycin, DAPI, Hoechst 33342). DNA histograms obtained in this way are comparable to those using conventional staining techniques, e.g., ethanol fixation followed by staining. Using this procedure the DNA content distribution of solid tissue or cells from suspension or monolayer cultures can be generated in less than 5 min.
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267 articles.
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1. Fluorochromes That Bind Nucleic Acids;Flow Cytometry Today;2022
2. A OneStep Solution to Fix and Stain Cells for Correlative Live and Fixed Microscopy;Current Protocols;2021-11
3. Hoechst 33342;Handbook of Fluorescent Dyes and Probes;2015-05-15
4. Ethidium bromide (Homidium bromide);Handbook of Fluorescent Dyes and Probes;2015-05-15
5. Propidium iodide (PI);Handbook of Fluorescent Dyes and Probes;2015-05-15