Studio Delle Sostanze di Origine Tumorale Ad Azione Inibente Sulla Catalasi Epatica «in Vivo» : Nota 3 - Isolamento da liquidi ascitici di frazioni proteiche aventi attività anticatalasica

Abstract

Two protein fractions, decreasing the liver catalase activity «in vivo», have been prepared from ascitic fluids of tumoral and non tumoral origin, by adsorption on calcium phosphate gel at pH 5, and desorption at pH 8. They are: a globulin fraction, that remains in the ascitic fluid after adsorpitin at pH 5; an albumin fraction that is desorbed at pH 8. The activity of the globulin fraction is due for the most part to low molecular weight substances which are adsorbed to the protein and may be separated from their protein support by dialysis. On the contrary the activity of the albumines has to be ascribed to the protein molecule. Denaturation does not affect the anticatalase activity « in vivo » of albumines, on the contrary it destroys the « in vitro » activity, which is present in the undenatured material.