« Influenza Degli Ormoni Sessuali Sulla Crescita Dell'Adenocarcinoma Mammario Spontaneo Del Topo in Vitro »

Author:

Monesi Valerio1

Affiliation:

1. (dal Centro di Oncologia Sperimentale del C.N.R., Sez. di Milano, diretta dal professor P. Rondoni e dalla Divisione di Anatomia ed Istologia Patologica dell'Istituto per lo Studio e la Cura dei Tumori di Milano, diretta dal professor C. Sirtori)

Abstract

Explants from spontaneous mammary adenocarcinoma of C 3 H and IV B mice were treated « in vitro » with oestrone, progesterone and testosterone. The « roller-tubes » technique was used for the observation of the migration area, the « roller-slides » technique for histological preparations and mitotic index calculations, the Carrel flasks for phase contrast microscopical observations. Growth determination was carried out in 15 days old cultures by planimetric measurements of the migration area and calculations of the mitotic index. 1) Cultured tumor cells show a tendency to convert from round into fusiform shape, increasing considerably in size and assuming a more differentiated aspect (Caspersson's « B » cells). The tendency to cytological differentiation is accompanied with a gradual reduction of the proliferation. 2) Oestrone administration enhances considerably the growth (increase of the migration area to more than the 400 per cent) and the tendency to the cyto-histological differentiation of the tumor. The stimulating effect of oestone is exerted only on epithelial elements. 3) Progesterone administration brings about a lower increase of the growth (augment of the migration area of the 308 per cent at a dilution of gr. 1.100-5/cc. and of the 39 per cent at a dilution of gr. 5.10-5/cc.) and of the tendency to cyto-histological differentiation of the tumour. In contrast with what observed for oestrone, the stimulating effect would be manifest also on the stroma. 4) Testosterone administration inhibits strongly the tumour growth of the 66 per cent at a dilution of gr. 1.10-5/cc and of the 29 per cent at a dilution of gr. 5.10-5/cc.) without influencing its differentiation. The stroma seems to be scarcely influenced by hormonal treatment. The inhibiting action of testosterone is accompanied by regressive phenomena of the tumour elements. Results show that the spontaneous adenocarcinoma of mouse maintains « in vitro » the hormono-dependency properties which are characteristic of the normal mammary cell.

Publisher

SAGE Publications

Subject

Cancer Research,Oncology,General Medicine

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3