Standardization of the Alkaline Elution Procedure Using X-Ray-Damaged Nuclear DNA

Author:

Bolognesi Claudia1,Cesarone Carmelo Federico1,Santi Leonardo1

Affiliation:

1. (Institute of Oncology, School of Medicine, University of Genova)

Abstract

Structural modifications of DNA were induced by X-irradiation of crude hepatic nuclei at various dose ranges to standardize DNA damage evaluated by the alkaline elution technique. This quantitative assay can be used as reference for DNA damage induced by the in vivo administration of mutagens and/or carcinogens involved in the environment.

Publisher

SAGE Publications

Subject

Cancer Research,Oncology,General Medicine

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