Quantitative Determination of Mammary Tumor Virus in Individual Samples of Mouse Milk-Reference-Cited by-同舟云学术

Quantitative Determination of Mammary Tumor Virus in Individual Samples of Mouse Milk

Published:1977-11 Issue:6 Volume:63 Page:525-534
ISSN:0300-8916
Container-title:Tumori Journal
language:en
Short-container-title:Tumori

Author:

Bistocchi Maria¹,Bevilacqua Generoso²,Nuti Marianna¹²

Affiliation:

1. Cattedra di Biochimica Applicata, Facoltà di Farmacia, Università, Pisa

2. Laboratory of Experimental Oncology, Istituto di Anatomia e Istologia Patologica, Facoltà di Medicina, Università, Pisa

Abstract

The possibility to determine quantitatively the intact mammary tumor virus (MTV) in milk of mice carrying milk-transmitted MTV has been assayed by a method that allows direct comparison between individual milk samples. The method is based on (a) the measure of light scattering of partially purified MTV preparations, (b) the use of milk from genetically identical MTV free mice as blank and (c) the quantitative reference to the total protein content of whole milk. The sensitivity, specificity and reproducibility of the procedure, as well as the requirement of appropriate quantitative references, are illustrated and discussed. BALB/c (MTV free), BALB/cfC3H, and BALB/cfRIII mice have been used.

Publisher

SAGE Publications

Subject

Cancer Research,Oncology,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/030089167706300605

Reference9 articles.

1. Quantitative Studies of Oncornaviruses in Thin Sections

2. Separation of B and C Type Virions by Centrifugation in Gentle Density Gradients

3. SheffieldJ. B., DalyT. M., TaraschiN., MooreD. H.: A comparison of the surface components of MuMTV from different sources. 10th Meeting on Mammary Cancer in Experimental Animals and Man. Abstracts, pp. 115. Kobe, Japan, 1976.

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