Possible Involvement of bcl-2 Suppression in Wild-Type p53 Gene-Dependent Cell Growth Repression in Rat Osteosarcoma Cells-Reference-Cited by-同舟云学术

Possible Involvement of bcl-2 Suppression in Wild-Type p53 Gene-Dependent Cell Growth Repression in Rat Osteosarcoma Cells

Published:2000-07 Issue:4 Volume:28 Page:575-579
ISSN:0192-6233
Container-title:Toxicologic Pathology
language:en
Short-container-title:Toxicol Pathol

Author:

Honoki Kanya¹,Tsujiuchi Toshifumi²,Tsutsumi Masahiro²,Kido Akira¹,Morishita Toru¹,Yoshimoto Masatoshi¹,Miyauchi Yoshizumi¹,Mii Yoshio¹,Tamai Susumu¹,Konishi Yoichi²

Affiliation:

1. Department of Orthopedic Surgery, Nara Medical University, Kashihara, Nara, Japan

2. Department of Oncological Pathology, Nara Medical University, Kashihara, Nara, Japan

Abstract

We recently obtained 3 cloned cell lines demonstrating the p53 mutation from a lung metastatic nodule of a rat transplantable osteosarcoma. In this study, we applied wild-type p53 gene transfer to the rat osteosarcoma cells by lipofection to investigate the effects on cell growth, expression of genes such as waf1/p21, bcl-2, and bax, and nucleosomal DNA fragmentation due to apoptosis. Reconstitution of the p53 gene inhibits cellular growth, and this growth-suppressive effect is partly due to apoptosis involving bcl-2 gene suppression in this tumor type. This rat osteosarcoma model is similar in biologic behavior to human cases and thus is very suitable for further investigation of tumorigenesis and gene therapy for osteosarcoma.

Publisher

SAGE Publications

Subject

Cell Biology,Toxicology,Molecular Biology,Pathology and Forensic Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/019262330002800411

Reference32 articles.

1. Cell proliferation, DNA repair, and p53 function are not required for programmed death of prostatic glandular cells induced by androgen ablation.

2. Bcl-2 blocks p53-dependent apoptosis.

3. Thymocyte apoptosis induced by p53-dependent and independent pathways

4. Osteogenic Sarcoma

5. p53 functions as a cell cycle control protein in osteosarcomas.

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