A Primary Hepatic Malignant Mesenchymal Tumor with Myofibrogenic Differentiation in a B6C3F1 Mouse

Abstract

We found a malignant mesenchymal tumor with myofibrogenic differentiation in the liver of a 110-week-old female B6C3F1 mouse used for a carcinogenicity study. Sclerous white patches (maximum size: 20 × 14 mm) were observed mainly in the median lobe of the liver at necropsy. Histologically, the tumor was composed of interlacing fascicles of spindle-shaped cells with oval or elongated nuclei and lightly eosinophilic cytoplasm. Tumor cells metastasized to the lung, parapancreatic lymph node, and spleen. Special staining revealed individual tumor cells surrounded by reticulin fibers and an abundant collagenous matrix. Immunohistochemically, the tumor cells were positive for vimentin, α-smooth muscle actin, and desmin, but were negative for von Willebrand factor, Mac-2, S-100, and cytokeratin. Electron microscopic examination revealed that the tumor cells contained prominent rough endoplasmic reticulum and thin filaments in the cytoplasm, although they lacked basal lamina, focal densities, or lipid droplets. Collagenous fibers were observed in the intercellular matrix. Thus, detailed histopathological examination suggested the origin of the present tumor to possibly be Ito cells within the fibrous stroma. This report provides additional histopathological evidence of malignant hepatic nonepithelial tumors in mice.