LOCALIZATION OF MITOCHONDRIAL ADENOSINE TRIPHOSPHATASE ACTIVITY IN CULTURED HUMAN CELLS-Reference-Cited by-同舟云学术

LOCALIZATION OF MITOCHONDRIAL ADENOSINE TRIPHOSPHATASE ACTIVITY IN CULTURED HUMAN CELLS

Published:1965-11 Issue:8 Volume:13 Page:647-656
ISSN:0022-1554
Container-title:Journal of Histochemistry & Cytochemistry
language:en
Short-container-title:J Histochem Cytochem.

Author:

ESSNER EDWARD¹,FOGH JØRGEN¹,FABRIZIO PATRICIA¹

Affiliation:

1. Divisions of Cytology and Experimental Pathology, Sloan-Kettering Institute for Cancer Research, New York, New York, and the Sloan-Kettering Division, Cornell University Medical College, New York, New York

Abstract

Adenosine triphosphatase activity has been localized by light microscopy in the mitochondria of four different types of cultured cells using the Wachstein-Meisel lead method after brief fixation in formol-calcium. The resolutions of the method permits the study of mitochondrial size, form, number and distributions in these cultured cells. Electron microscopy shows enzyme reaction product within the mitochondrion but not on the outer mitochondrial membrane. Reaction product is also localized to the plasma membrane and its infoldings.

Publisher

SAGE Publications

Subject

Histology,Anatomy

Link

http://journals.sagepub.com/doi/pdf/10.1177/13.8.647

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